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Journal: The Journal of Biological Chemistry
Article Title: The retaining kdo transferase that synthesizes Escherichia coli K13 capsule is deeply divergent from structurally homologous enzymes
doi: 10.1016/j.jbc.2026.111162
Figure Lengend Snippet: Reaction catalyzed by KrkA GT140 . A , TLC analysis of KrkA GT 140 reaction containing Kdo, CTP, acceptor 1, KdsB and KrkA GT 140 . Reaction reached completion after 15 min. B , HPLC trace for the reaction of KrkA GT 140 ( black ) and acceptor 1 ( green ) showing that the product formed eluted at around 26 min. C , mass spectrum of the KrkA GT 140 reaction showing the single addition of Kdo as the major peak ( green highlight ).
Article Snippet:
Techniques:
Journal: The Journal of Biological Chemistry
Article Title: The retaining kdo transferase that synthesizes Escherichia coli K13 capsule is deeply divergent from structurally homologous enzymes
doi: 10.1016/j.jbc.2026.111162
Figure Lengend Snippet: Overall structure of KrkA GT 140 . A and B , orthogonal views of the structure of the protomer, with domains and secondary structure elements as indicated. CMP is shown as yellow sticks. C , topology diagram showing the overall organization of the protein. D, structure of the trimer. E , superposition of WbbB (8mgd, in white ) on the KrkA GT 140 protomer (multicolored).
Article Snippet:
Techniques:
Journal: The Journal of Biological Chemistry
Article Title: The retaining kdo transferase that synthesizes Escherichia coli K13 capsule is deeply divergent from structurally homologous enzymes
doi: 10.1016/j.jbc.2026.111162
Figure Lengend Snippet: CMP binding, and model of CMP-β-Kdo binding. A , CMP binding in the WT KrkA GT 140 binding site. B , model of CMP-β-Kdo binding. The structure of WbbB with CMP-β-Kdo bound (8csb) was superimposed on KrkA GT 140 , and then manually adjusted.
Article Snippet:
Techniques: Binding Assay